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Limiting inclusion to trials that called for at least 1000 mg/day made no difference.
Early symptoms are malaise and lethargy, progressing to shortness of breath, bone pain, bleeding gums, susceptibility to bruising, poor wound healing, and finally fever, convulsions and eventual death.
Two meta-analyses evaluated the effect of vitamin C supplementation on the risk of colorectal cancer.
One found a weak association between vitamin C consumption and reduced risk, and the other found no effect of supplementation.
Doing so converts vitamin C to an oxidized state - either as semidehydroascorbic acid or dehydroascorbic acid.
These compounds can be restored to a reduced state by glutathione and NADPH-dependent enzymatic mechanisms.